mouse lymphocyte subset antibody cocktail  (Elabscience Biotechnology)

Journal: Vaccines

Article Title: Immunogenicity of an Inactivated Senecavirus A Vaccine with a Contemporary Brazilian Strain in Mice

doi: 10.3390/vaccines12080845

Figure Lengend Snippet: Panel of fluorochrome-labeled monoclonal antibodies.

Article Snippet: Mouse T Lymphocyte Subset Antibody Cocktail (cod 558431, BD Becton Dickinson) , anti-CD3e anti-CD4 anti-CD8α , PE-Cy™7 PE APC.

Techniques: Activation Assay

Journal: Virology Journal

Article Title: Immunological profile of mice immunized with a polyvalent virosome-based influenza vaccine

doi: 10.1186/s12985-023-02158-0

Figure Lengend Snippet: Panel of fluorochrome-labeled monoclonal antibodies

Article Snippet: Mouse B Lymphocyte Subset Antibody Cocktail (cod 558,332, BD Becton Dickinson) , anti-CD45R / B220 / PE-Cy™7 anti-CD23 (FcεRII) / PE anti-sIgM / APC.

Techniques: Activation Assay

Journal: Journal of Immunology Research

Article Title: Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa

doi: 10.1155/2018/3835720

Figure Lengend Snippet: Mincle, Dectin-1, and Dectin-2 agonists induce comparable antigen-specific CD4 + and CD8 + T cell response. Mice were immunized twice by subcutaneous injection of ovalbumin alone or in combination with TDB, curdlan, or furfurman. Naïve mice were used as negative control. Splenocytes were harvested 14 days after the second immunization, labeled with CFSE, restimulated with 1 μ g/mL ovalbumin for 72 h, stained with fluorescently tagged antibodies against CD3, CD4, and CD8, and analyzed by flow cytometry. (a) Gating strategy to quantify proliferating CD4 + and CD8 + T cells. (b) Minimum, maximum, and median of the abundance of ovalbumin-specific proliferating CD4 + and CD8 + T cells from two independent experiments with 5 mice per group per experiment. Data were compared using the two-tailed unpaired Mann–Whitney t -test. # p < 0.05; # # p < 0.01 relative to control group. ∗ p < 0.05; ∗∗ p < 0.01 between Mincle-, Dectin-1-, and Dectin-2-stimulated cells.

Article Snippet: 72 hours later, cells were harvested, washed in PBS, and then stained using BD Pharmingen Mouse T-Lymphocyte Subset Antibody Cocktail (anti-CD3, anti-CD4, and anti-CD8) with corresponding isotype controls for 20 min at 4°C, in Staining Buffer (all, BD Biosciences, USA) according to the manufacturer's instructions.

Techniques: Injection, Negative Control, Labeling, Staining, Flow Cytometry, Two Tailed Test, MANN-WHITNEY